Scottish Marine and Freshwater Science Vol 11 No 6
This report describes the method validation for GC-MS quantification of n-alkanes using deuterated internal standards to determine the presence of hydrocarbons in the environment following an oil spill. A gas chromatography – mass spectrometry (GC-MS) method was developed and validated for the determination of n-alkanes and isoprenoids (pristane and phytane) in fish (trout muscle), using seven deuterated internal standards. Data are presented in csv files alongside the report.
Data and Resources
| Field | Value |
|---|---|
| Publisher | |
| Modified | 2020-07-09 |
| Release Date | 2020-07-09 |
| Identifier | e544ecd0-402e-4c77-84fe-a5a6f34c7b93 |
| Spatial / Geographical Coverage Location | Scotland |
| License | UK Open Government Licence (OGL) |
| Data Dictionary | Reflux saponification coupled with liquid-liquid extraction was utilised for the extraction of n-alkanes. Clean up and fractionation was performed in order to minimise interferences and obtain the aliphatic fraction from the extract. Chromatographic determination by GC-MS was applied to the separation and identification of n-C10 to n-C35, as well as pristane and phytane. The newly developed method was validated and compared to a pre-existing method used by Marine Scotland Science (MSS) based on a two-component internal standard containing squalane and heptamethylnonane (HMN). |
| Contact Name | Marine Scotland |
| Contact Email | |
| Public Access Level | Public |