Scottish Marine and Freshwater Science Vol 11 No 6
This report describes the method validation for GC-MS quantification of n-alkanes using deuterated internal standards to determine the presence of hydrocarbons in the environment following an oil spill. A gas chromatography – mass spectrometry (GC-MS) method was developed and validated for the determination of n-alkanes and isoprenoids (pristane and phytane) in fish (trout muscle), using seven deuterated internal standards. Data are presented in csv files alongside the report.
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Reflux saponification coupled with liquid-liquid extraction was utilised for the extraction of n-alkanes. Clean up and fractionation was performed in order to minimise interferences and obtain the aliphatic fraction from the extract. Chromatographic determination by GC-MS was applied to the separation and identification of n-C10 to n-C35, as well as pristane and phytane. The newly developed method was validated and compared to a pre-existing method used by Marine Scotland Science (MSS) based on a two-component internal standard containing squalane and heptamethylnonane (HMN).
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