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monitoring

Marine Scotland Science (MSS) undertakes a wide range of monitoring covering many aspects of the Scottish marine ecosystem.

Monitoring is undertaken in Scottish Inshore Waters, using networks of volunteers and automatic recording equipment, as well as in Scottish Offshore Waters, using the MSS research vessels MRV Alba na Mara and MRV Scotia.

Marine Scotland Science also participates national monitoring programmes, such as:

  • UK Marine Monitoring and Assessment Strategy (UKMMAS)
  • UK Marine Environmental Change Network (MECN)
  • UK Marine Environmental Data and Information Network (MEDIN)

This group provides published data from monitoring these activities. For many activities, there are national or international standards for how the monitoring is undertaken.

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UK Open Government Licence (OGL)

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Microarray for the Detection and Quantification of Toxin-Producing Phytoplankton Species in Scottish Coastal Waters

doi: 
10.7489/2019-1

Scottish Marine and Freshwater Science Vol 8 No 24

A small number of phytoplankton species have the ability to produce algal toxins which can accumulate in filter feeding bivalves such as oysters, scallops and mussels. The bioaccumulation of algal toxins can potentially cause serious health issues to shellfish consumers. Countries with a shellfish aquaculture industry (such as Scotland) have set up for their classified production areas a phytoplankton monitoring programme as part of their legal obligations (EC 854/2004 and amendments). This involves the regular collection of water samples to assess the phytoplankton community using light microscopy, with a particular emphasis on the toxin producing species. However, this technique lacks the ability to identify some key phytoplankton to a species level (e.g. Pseudo-nitzschia sp., Alexandrium sp.), which is critical to appropriately assess the potential toxicity of a phytoplankton bloom event. The objective of this project was to evaluate a microarray technique which was developed during the MIDTAL project (Microarrays For The Detection of Toxic Algae: http://www.midtal.com/) to identify phytoplankton to species level. Microarrays are modified glass supports on which are printed RNA probes that are species-specific. Each targeted phytoplankton species is defined by a set of probes that are statistically unique to each one. For the purpose of this project, water samples were collected offshore from Stonehaven (East coast of Scotland) over a two years period (starting in 2015) and processed using the microarray to assess its specificity and sensitivity in relation to the identification and potential semi-quantification of toxic strains of phytoplankton.

Citation: 
Hermann, G., Graham, J. and Lacaze, J-P. (2017) Microarray for the Detection and Quantification of Toxin-Producing Phytoplankton Species in Scottish Coastal Waters. Scottish Marine and Freshwater Science Vol 8 No 24, DOI: 10.7489/2019-1
FieldValue
Publisher
Modified Date
2017-12-15
Release Date
2017-12-15
Identifier
3e6d291f-5097-4755-9d13-17118044a0d2
Spatial / Geographical Coverage Location
Scotland
Temporal Coverage
Thursday, January 1, 2015 - 00:00 to Saturday, December 31, 2016 - 00:00
License
UK Open Government Licence (OGL)
Author
G. Hermann
Data Dictionary

Integrated water samples were collected weekly (25/03/2015 - 19/09/2016) at Stonehaven (about three miles offshore) using a 10 m tube sampler. The contents of each integrated water sample were mixed into a 10 L carboy before 1 L aliquots were taken for light microscopy analysis and microarray processing. For the microarray, each aliquoted sample was filtered through a 1 µm, 25 mm nitrocellulose filter. The filter was then soaked in 1 mL of Tri-reagent and stored at -80°C until RNA extraction. In total, 72 phytoplankton samples were processed using that protocol. Another water sample aliquot was also processed for light microscopy analysis using the Utermöhl method (Utermöhl, 1931) to estimate the phytoplankton community.

Contact Name
Marine Scotland Science
Contact Email
Public Access Level
Public