{"help":"Return the metadata of a dataset (package) and its resources. :param id: the id or name of the dataset :type id: string","success":true,"result":[{"id":"c3fe6152-88ae-4d03-8ce1-c5110092925e","name":"development-and-validation-high-performance-liquid-chromatography-method-quantify","title":"The Development and Validation of a High Performance Liquid Chromatography Method to Quantify Chlorophylls a and b in Marine Phytoplankton and Identify Other Pigments of Interest ","author":"K Smith, L Webster, E Bresnan and C F Moffat","author_email":"ms.reception@scotland.gsi.gov.uk","maintainer":"Scottish Government - Marine Directorate Data Publications","maintainer_email":"marinedirectorate@gov.scot","notes":"\u003Cp\u003EScottish Marine and Freshwater Science Volume 1 No 12\u003C\/p\u003E\n\u003Col\u003E\n\u003Cli\u003E\n\u003Cp\u003EHigh Performance Liquid Chromatography (HPLC) is an alternative method to light  microscopy and fluorescence for characterising phytoplankton. The HPLC data  provides valuable information about the contribution of different functional groups to  the biomass of the phytoplankton community. The HPLC data should be supported  by microscopic verification as many algal classes share the same pigments.\u003C\/p\u003E\n\u003C\/li\u003E\n\u003Cli\u003E\n\u003Cp\u003EA quantative HPLC method was developed to quantify chlorophyll a and chlorophyll  b. However, due to a lack of commercially available standards at suitable  concentrations, only area % data is given for 19 hexanoyloxyfucoxanthin,  alloxanthin, alpha-carotene, antheraxanthin, beta-carotene, chlorophyll c2, chlorophyll  c3, diadinoxanthin, diatoxanthin, fucoxanthin, gyroxanthin-diester, lutein, neoxanthin,  peridinin, prasinoxanthin, violaxanthin and zeaxanthin.\u003C\/p\u003E\n\u003C\/li\u003E\n\u003Cli\u003E\n\u003Cp\u003EAmmonium acetate buffer (0.5 mol l-1) is added to the standards and samples  immediately before injection, in order to increase the affinity of the pigments for the  column during the loading step. This reaction is temperature dependant and must be  carried out at ambient temperature to ensure adequate mixing.\u003C\/p\u003E\n\u003C\/li\u003E\n\u003Cli\u003E\n\u003Cp\u003EThe stability of the pigments, in the autosampler at ambient temperature, whilst  awaiting injection for a typical analytical run, was investigated. All pigments were  found to be stable in the autosampler for 18 hours. A typical analytical batch takes  15 hours of analytical time.\u003C\/p\u003E\n\u003C\/li\u003E\n\u003Cli\u003E\n\u003Cp\u003EReplicate analysis for high standards and samples and low standards and samples  on different days gave %CV of \u0026lt;11% for both chlorophyll a concentration and  chlorophyll b concentration. This meets the limits for reproducibility given in the UK  Clean Seas Environmental Monitoring Programme (CSEMP) Green Book. 2\u003C\/p\u003E\n\u003C\/li\u003E\n\u003Cli\u003E\n\u003Cp\u003EThe limit of detection (LOD) was calculated to be 0.1 \u00b5g l-1 for chlorophyll a and 0.02  \u00b5g l-1 for chlorophyll b. This meets the limits for LOD given in CSEMP Green Book.\u003C\/p\u003E\n\u003C\/li\u003E\n\u003Cli\u003E\n\u003Cp\u003ERecovery for chlorophyll a was calculated to be 105%, and 110% for chlorophyll b,  which meets the limits set by the United Kingdom National Marine Chemistry  Advisory Group (NMCAG).\u003C\/p\u003E\n\u003C\/li\u003E\n\u003C\/ol\u003E\n","url":"https:\/\/data.marine.gov.scot\/dataset\/development-and-validation-high-performance-liquid-chromatography-method-quantify","state":"Active","log_message":"Update to resource \u0027Property title\u0027","private":true,"revision_timestamp":"Tue, 01\/07\/2020 - 17:22","metadata_created":"Tue, 09\/02\/2014 - 13:14","metadata_modified":"Tue, 01\/07\/2020 - 17:22","creator_user_id":"30436a8d-34e8-47d5-b586-8a555ab42696","type":"Dataset","resources":[{"id":"22b16ce7-138e-469f-b081-2f758ac6e2f9","revision_id":"","url":"http:\/\/www.scotland.gov.uk\/Resource\/Doc\/295194\/0111313.pdf","description":"","format":"data","state":"Active","revision_timestamp":"Tue, 01\/07\/2020 - 14:53","name":"Scottish Marine and Freshwater Science Volume 1 No 12","mimetype":"data","size":"","created":"Tue, 09\/02\/2014 - 13:14","resource_group_id":"d0fc86dc-1394-43d4-aef1-c8cf5ac500f9","last_modified":"Date changed  Tue, 01\/07\/2020 - 14:53"}],"tags":[{"id":"40f77792-ba01-4920-ab5f-24c534128669","vocabulary_id":"2","name":"HPLC"},{"id":"c319b4a8-fc51-4658-a7e5-4a6a2e1c6092","vocabulary_id":"2","name":"Pigments"}],"groups":[{"description":"\u003Cp\u003EFormal report series, containing results of research and monitoring carried out by Marine Directorate.\u003C\/p\u003E\n","id":"d0fc86dc-1394-43d4-aef1-c8cf5ac500f9","image_display_url":"https:\/\/data.marine.gov.scot\/sites\/default\/files\/\/reports_1.png","title":"Scottish Marine and Freshwater Science Reports","name":"group\/scottish-marine-and-freshwater-science-reports"}]}]}